Research and Studies

TOXICOLOGY AND SAFETY STUDIES
-Mutagenicity Test (Ames Test) LENTIN PLUS 1000, up to a concentration of 10,000 μg/plate, tested negative in a bacterial reverse mutation test, both with and without metabolic stimulation. Conducted by Consumer Product Testing Company, NJ, USA
-Single Dose Test in Rats Male and female Wistar rats were given a single oral dose of LENTIN PLUS 1000 at 5, 10, 18, 27, or 36 g/kg. LENTIN PLUS 1000 was regarded as non-toxic according to the reference: LD50>36 g/kg. Conducted by AMA Laboratories, Inc. NY, USA
-Repeated Dose Test in Dogs Ina safety study of LENTIN PLUS 1000 in dogs, male and female beagle dogs were treated with 0, 20, or 200 mg/kg of LENTIN PLUS 1000, admixed to their feed once daily for a period of 4 weeks (28 doses). Even after the administration of LENTIN PLUS 1000 for 4 weeks at a dose of 20 mg/kg/day or 200 mg/kg/day, no abnormal findings were observed in general conditions, body weight, food intake, hematology, or blood biochemistry. In this study, the No Observed Adverse Effect Level (NOAEL) of the product was estimated to be 200 mg/kg/day or more. Conducted by Kyodoken Institute. Kyoto, Japan
-Human Studies Subjects participated in a two-group, randomized intervention, where one group (n=10) consumed 1 gram/day and the other (n=10) consumed 3 gram/day. Safety and tolerability of LENTIN PLUS 1000 were assessed with total bilirubin, total protein, creatinine, and liver function tests.
All subjects tolerated the product without any adverse reactions. Ali KH, et. al., “An Open-label, Randomized Clinical Trial to Assess the Immunomodulatory Activity of a Novel Oligosaccharide Compound in Healthy Adults”, Functional Foods in Health and Disease. 2(7):265-279.,2012.

 

List of published research

YearTitle AuthorJournal
2016From Bench to Bedside: The Growing Use of Arabinoxylan Rice Bran (MGN-3/BioBran) in Cancer ImmunotherapyGhoneum M.Austin Immunol. 2016: 1(2): 1006
2016Arabinoxylan rice bran (BioBran) suppresses the viremia level in patients with chronic HCV infectionHosny Salama, Eman Medhat, Magda Shaheen, Abdel-Rahman N Zekri, Tarneem Darwish, and Mamdooh GhoneumInternational Journal of Immunopathology and Pharmacology 1-7
2016Addition of Rice Bran Arabinoxylan to Curcumin Therapy May Be Benefit to Patients with Early-Stage B-Cell Lymphoid Malignancies (Monoclomal Gammopathy of Undetermined Significance, Smoldering Multiple Myeloma, or Stage 0/1 Chronic Lymphocytic Leukemia): ATerry Golombick, Terrence H. Diamond, Arumugam Manoharan, and Rajeev RamakrishnaIntegrative Cancer Therapies 2016
2016Can the EGFR inhibitors increase the immunomodulatory effects of standardized plant extracts (mistletoe lectin and arabonoxylan) with clinical benefit? Case report of a patient with lung adenocarcinoma)Tibor Hajto, Anna Horvath, Lilla Baranyai, Monika Kuzma and Pal PerjesiClin Case Rep Rev, 2016 Volume 2(6): 456-459 doi: 10.15761/CCRR.1000244
2016Chemopreventive Activity of MGN-3/Biobran Against Chemical Induction of Glandular Stomach Carcinogenesis in Rats and Its Apoptotic Effect in Gastric Cancer Cells.

Badr El-Din NK, Abdel Fattah SM, Pan D, Tolentino L, Ghoneum M.Integr Cancer Ther. 2016 May 4. pii: 1534735416642287
2016Improvement of Quality of Life in Tumor Patients after an Immunomodulatory Treatment with Standardized Mistletoe Lectin and Arabinoxylan Plant Extracts.

Hajto T, Horváth A, Papp S.International Journal of Neurorehabilitation Vol.3 doi:10.4172/2376-0281.1000205, 2016
2015Can a synergistic activation of pattern recognition receptors by plant immunomodulators enhance the effect of oncologic therapy? Case Report of a patient with uterus and ovary sarcoma.Hajto T, Baranyai L, Kirsch A, Kuzma M, Perjési P.Clin Case Rep Rev. 2015 1 (10): 235-8.
2014Mgn-3/biobran enhances generation of cytotoxic CD8+ T cells via upregulation of dec-205 expression on dendritic cells.Ghoneum M, Agrawal S.Int J Immunopathol Pharmacol. 2014 Oct-Dec;27(4):523-30.
2014Arabinoxylan rice bran (MGN-3/Biobran) enhances natural killer cell-mediated cytotoxicity against neuroblastoma in vitro and in vivo.Pérez-Martínez A, Valentín J, Fernández L, Hernández-Jiménez E, López-Collazo E, Zerbes P, Schwörer E, Nuñéz F, Martín IG, Sallis H, Díaz MÁ, Handgretinger R, Pfeiffer MM.Cytotherapy. 2014 Dec 22.
2014Therapeutic Effects of BioBran, Modified Arabinoxylan Rice Bran, in Improving Symptoms of Diarrhea Predominant or Mixed Type Irritable Bowel Syndrome: A Pilot, Randomized Controlled StudyKamiya T, Shikano M, Tanaka M, Ozeki K, Ebi M, Katano T, Hamano S, Nishiwaki H, Tsukamoto H, Mizoshita T, Mori Y, Kubota E, Tanida S, Kataoka H, Okuda N, Joh T.Evid Based Complement Alternat Med. 2014;2014:828137
2014Modified Arabinoxylan from Rice Bran, MGN-3/BioBran, Sensitizes Metastatic Breast Cancer Cells to Paclitaxel in VitroGhoneum M, Badr El-Din NK, Ali DA, El-Dein MAAnticancer Res. 2014 Jan;34(1):81-7
2013BIOBRAN MGN-3: Effect of reducing side effects of chemotherapy in breast cancer patientsDr. Ahmad Ijaz Masood, Dr Rabeeta Sheikh, Dr Rana Atique AnwerThe Professional Medical Journal Jan-Feb 2013;20(1): 013-016
2013Case Reports of Cancer Patients with Hepatic Metastases Treated by Standardized Plant Immunomodulatory PreparationsHajto T, Kirsch AJournal of Cancer Research Updates. 2013;2:1-9
2013A case of stage IV hepatocellular carcinoma treated by KM900, BioBran, and psychotherapy has presented significant good resultsMeshitsuka K.Personalized Medicine Universe. 2013.1 (1): 46-8.
2013Arabinoxylan Rice Bran (MGN-3/BioBran) Provides Protection against Whole-body γ-irradiation in Mice via Restoration of Hematopoietic TissuesGhoneum M, Badr El-Din NK, Abdel Fattah SM, Tolentino LJ Radiat Res. 2013 May;54(3):419-29
2013MGN-3 Arabinoxylan Rice Bran Modulates Innate Immunity in Multiple Myeloma PatientsCholujova D, Jakubikova J, Czako B, Martisova M, Hunakova L, Duraj J, Mistrik M, Sedlak JCancer Immunol Immunother. 2013 Mar;62(3):437-45
2012An Open-label, Randomized Clinical Trial to Assess The Immunomodulatory Activity of a Novel Oligosaccharide Compound in Healthy AdultsAli KH, Melillo AB, Leonard SM, Asthana D, Woolger JM, Wolfson AH, McDaniel HR, Lewis JEFunctional Foods in Health and Disease. 2012; 2(7) 265-79
2012Suppressive Effect of Modified Arabinoxylan from Rice Bran (MGN-3) on D-galactosamine-induced IL-18 Expression and Hepatitis in RatsZheng S, Sanada H, Dohi H, Hirai S, Egashira YBiosci Biotechnol Biochem. 2012;76(5):942-6
2011Synergistic Apoptotic Effect of Arabinoxylan Rice Bran (MGN-3/BioBran) and Curcumin (Turmeric) on Human Multiple Myeloma Cell Line U266 in VitroGhoneum M, Gollapudi SNeoplasma. 2011;58(2):118-23
2011Activation of Human Monocyte-derived Dendritic Cells in Vitro by The Biological Response Modifier Arabinoxylan Rice Bran (MGN-3/BioBran)Ghoneum M, Agrawal SInt J Immunopathol Pharmacol. 2011 Oct-Dec;24(4):941-8
2010Arabinoxylan Rice Bran (MGN-3) Enhances The Effects of Interventional Therapies for The Treatment of Hepatocellular Carcinoma: A Three-year Randomized Clinical TrialBang MH, Van Riep T, Thinh NT, Song le H, Dung TT, Van Truong L, Van Don L, Ky TD, Pan D, Shaheen M, Ghoneum MAnticancer Res. 2010 Dec;30(12):5145-51.
2009Functionality of BioBran (Rice Bran Arabinoxylan – Focusing on the Promotion Effect of Dendritic Cell MaturationKato H, Mihara SNew Food Industry. 2010;52(6) (1)
2009Epigenetics and Immunosenescence Reversal: An Evidence-Based Longevity ParadigmAli KHAnti-Aging Medical Therapeutics. 2009;6
2009BioBran-augmented Maturation of Human Monocyte-derived Dendritic CellsCholujova D, Jakubikova J, Sedlak JNeoplasma. 2009;56(2):89-95
2008Modified Arabinoxylan Rice Bran (MGN-3/BioBran) Enhances Intracellular Killing of Microbes by Human Phagocytic Cells in VitroGhoneum M, Matsuura M, Gollapudi SInt J Immunopathol Pharmacol. 2008 Jan-Mar;21(1):87-95
2008In Vivo Tumor Inhibitory Effects of Nutritional Rice Bran Supplement MGN-3/BioBran on Ehrlich Carcinoma-bearing MiceBadr El-Din NK, Noaman E, Ghoneum MNutr Cancer. 2008;60(2):235-44
2008Antioxidant Potential by Arabinoxylan Rice Bran, MGN-3/BioBran, Represents a Mechanism for Its Oncostatic Effect against Murine Solid Ehrlich CarcinomaNoaman E, Badr El-Din NK, Bibars MA, Abou Mossallam AA, Ghoneum MCancer Lett. 2008 Sep 18;268(2):348-59
2008MGN-3/BioBran, Modified Arabinoxylan from Rice Bran, Sensitizes Human Breast Cancer Cells to Chemotherapeutic Agent, DaunorubicinGollapudi S, Ghoneum MCancer Detect Prev. 2008;32(1):1-6
2007Yeast Therapy for the Treatment of Cancer and Its Enhancement by MGN-3/BioBran, an Arabinoxylan Rice BranGhoneum M, Brown J, Gollapudi SCellular Signaling and Apoptosis Research. 2007;185-200
2006Metastatic Hemangiopericytoma of The Skin Treated with Wide Local Excision and MGN-3Markus J, Miller A, Smith M, Orengo IDermatol Surg. 2006 Jan;32(1):145-7
2005Synergistic Role of Arabinoxylan Rice Bran (MGN-3/BioBran) in S. Cerevisiae-induced Apoptosis of Monolayer Breast Cancer MCF-7 CellsGhoneum M, Gollapudi SAnticancer Res. 2005 Nov-Dec;25(6B):4187-96
2005Modified Arabinoxylan Rice Bran (MGN-3/BioBran) Enhances Yeast-induced Apoptosis in Human Breast Cancer Cells in VitroGhoneum M, Gollapudi SAnticancer Res. 2005 Mar-Apr;25(2A):859-70
2004Enhancement of Natural Killer Cell Activity of Aged Mice by Modified Arabinoxylan Rice Bran (MGN-3/BioBran)Ghoneum M, Abedi SJ Pharm Pharmacol. 2004 Dec;56(12):1581-8
2004Augmentation of Macrophage Phagocytosis by Modified Arabinoxylan Rice Bran (MGN-3/BioBran)Ghoneum M, Matsuura MInt J Immunopathol Pharmacol. 2004 Sep-Dec;17(3):283-92
2004The Life Prolongation and QOL Improvement Effect of Rice Bran Arabinoxylan Derivative (MGN-3, BioBran) for Progressive CancerTakahara K, Sano KClinical Pharmacology and Therapy. 2004;14(3):267-71
2004A Case Where an Immunomodulatory Food was Effective in Conservative Therapy for Progressive Terminal Pancreatic CancerKaketani KClinical Pharmacology and Therapy. 2004;14(3):273-9
2004One Case of a Patient with Umbilical Metastasis of Recurrental Cancer (Sister Mary Joseph’s Nodule, SMJN) Who has Survived for a Long Time under Immnomodulatory Supplement TherapyKawai TClinical Pharmacology and Therapy. 2004;14(3):281-8
2004The Clinical Significance of BioBran in The Immunotherapy for CancerOkamura YClinical Pharmacology and Therapy. 2004;14(3):289-94
2004Experience with Administration of BioBran in Patients with Chronic RheumatismIchihashi KClinical Pharmacology and Therapy. 2004;14(3):459-63
2003Modified Arabinoxylan Rice Bran (MGN-3/BioBran) Sensitizes Human T Cell Leukemia Cells to Death Receptor (CD95)-induced ApoptosisGhoneum M, Gollapudi SCancer Lett. 2003 Nov 10;201(1):41-9
2003Modified Rice Bran Beneficial for Weight Loss of Mice as a Major and Acute Adverse Effect of CisplatinEndo Y, Kanbayashi HPharmacol Toxicol. 2003 Jun;92(6):300-3
2002Modified Rice Bran Improves Glucose Tolerance in NIDDM Adult Rats Given Streptozotocin as NeonatesOhara I, Onai K, Maeda HStudies, Aichi Gakusen University. 2002;37:17-23
2001The Effect of MGN-3 on Cisplatin and Doxorubicin Induced Toxicity in The RatJacoby HI, Wnorowski G, Sakata K, Maeda HJournal of Nutraceuticals, Functional & Medical Foods. 2001;3(4):3-11
2001The Basic Study of Arabinoxylan Compound (MGN-3) on The Activation of Vital DefenseSudo N, Tatoe K, Koyama N, Kanna H, Hirayama K, Kubo CRinshou to Kenkyuu. 2001;78(1):193-196
2000Effects of Modified Rice Bran on Serum Lipids and Taste Preference in Streptozotocin-induced Diabetic RatsOhara I, Tabuchi R, Onai K, Econ MHNutrition Research.2000;20(1):59-68
2000Scavenging Activity of MGN-3 (Arabinoxylan from Rice Bran) with Natural Killer Cell Activity on Free RadicalsTazawa K, Namikawa H, Oida N, ltoh K, Yatsuzuka M, Koike J, Masada M, Maeda HBiotherapy. 2000;14(5):493-495
2000Production of Tumor Necrosis Factor-alpha and Interferon-gamma from Human Peripheral Blood Lymphocytes by MGN-3, a Modified Arabinoxylan from Rice Bran, and Its Synergy with Interleukin-2 in VitroGhoneum M, Jewett ACancer Detect Prev. 2000;24(4):314-24
1999NK Inmmunorestoration of Cancer patients by MGN-3, a Modified Arabinoxylane Rice Bran (Study of 32 Patients Followed up to 4 years)Ghoneum M, Brown JAnti-Aging Medical Therapeutics. 1999;3:217-26
1998Anti-HIV Activity in Vitro of MGN-3, an Activated Arabinoxylane from Rice BranGhoneum MBiochem Biophys Res Commun. 1998 Feb 4;243(1):25-9
1998Enhancement of Human Natural Killer Cell Activity by Modified Arabinoxylan from Rice Bran (MGN-3)Ghoneum MInternational Journal of Immunotherapy. 1998; XI V(2):89-99